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Related Experiment Videos

Glucocorticoids decrease a binding of corticotropin-releasing hormone-binding protein in human plasma.

T Suda1, T Sumitomo, Y Nakano

  • 1Department of Medicine, Tokyo Women's Medical College, Japan.

The Journal of Clinical Endocrinology and Metabolism
|October 1, 1990
PubMed
Summary

Glucocorticoids reduce corticotropin-releasing hormone-binding protein (CRH-BP) levels in plasma. This study investigated CRH-BP binding in patients with adrenal disorders, finding altered levels linked to cortisol concentrations.

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Age-dependent decline in cortisol levels and clinical manifestations in patients with ACTH-independent Cushing's syndrome.

Clinical endocrinology·1998

Area of Science:

  • Endocrinology
  • Hormone Research
  • Clinical Diagnostics

Background:

  • Corticotropin-releasing hormone-binding protein (CRH-BP) plays a role in regulating the hypothalamic-pituitary-adrenal (HPA) axis.
  • Plasma CRH-BP levels and binding are altered in various HPA disorders.

Purpose of the Study:

  • To investigate the binding of CRH-BP to human CRH in patients with HPA axis disorders.
  • To explore the relationship between CRH-BP binding, cortisol levels, and glucocorticoid treatment.

Main Methods:

  • Examined CRH-BP binding in plasma of patients with Cushing's syndrome, Addison's disease, and hypopituitarism.
  • Utilized labeled human CRH for binding assays.
  • Performed Scatchard analysis to assess binding affinity and site number.

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Main Results:

  • CRH-BP binding was decreased in Cushing's syndrome and with long-term high-dose glucocorticoid treatment.
  • CRH-BP binding was increased in Addison's disease and hypopituitarism.
  • Glucocorticoid replacement normalized increased CRH-BP binding in Addison's disease.
  • A negative correlation was observed between plasma cortisol and CRH-BP binding in Cushing's syndrome.
  • Scatchard analysis indicated reduced CRH-BP binding sites in Cushing's syndrome patients.

Conclusions:

  • Glucocorticoids appear to decrease CRH-BP binding in human plasma, likely by reducing CRH-BP concentration.
  • Altered CRH-BP binding is a characteristic of hypercortisolemia and other HPA axis dysregulations.