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Related Concept Videos

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...

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Updated: May 31, 2026

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
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Published on: May 2, 2019

Conjugated polymer nanoparticles for small interfering RNA delivery.

Joong Ho Moon1, Eladio Mendez, Yong Kim

  • 1Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th St., Miami, FL 33199, USA. jmoon@fiu.edu

Chemical Communications (Cambridge, England)
|June 23, 2011
PubMed
Summary

Conjugated polymer nanoparticles (CPNs) effectively deliver small interfering RNA (siRNA) for gene silencing. These nontoxic CPNs achieved 94% target gene down-regulation in HeLa cells, showing promise for therapeutic applications.

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Last Updated: May 31, 2026

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Porous Silicon Microparticles for Delivery of siRNA Therapeutics
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Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo
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Published on: February 5, 2019

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Molecular Biology

Background:

  • Gene silencing therapies require efficient and safe delivery vehicles.
  • Conjugated polymer nanoparticles (CPNs) offer potential for biomedical applications due to their tunable properties.
  • Visualizing the delivery process is crucial for optimizing nanocarrier performance.

Purpose of the Study:

  • To evaluate the efficacy and safety of CPNs as non-viral vectors for small interfering RNA (siRNA) delivery.
  • To demonstrate the gene silencing capability of CPNs/siRNA complexes in a cellular model.
  • To visualize the delivery process of CPNs within cells.

Main Methods:

  • Synthesis and characterization of loosely aggregated CPNs.
  • Complexation of CPNs with siRNA.
  • Transfection of HeLa cells with CPNs/siRNA complexes.
  • Quantification of gene down-regulation using appropriate assays.
  • Visualization of CPNs delivery using microscopy techniques.

Main Results:

  • CPNs were successfully synthesized and shown to be nontoxic.
  • CPNs effectively complexed with siRNA, forming stable nanoparticles.
  • Transfection with CPNs/siRNA complexes resulted in significant gene silencing (94% down-regulation of the target gene).
  • Delivery and intracellular localization of CPNs were visualized, confirming cellular uptake.

Conclusions:

  • Loosely aggregated CPNs are effective and nontoxic siRNA delivery vehicles.
  • CPNs demonstrate significant potential as carriers for gene silencing applications.
  • The ability to visualize delivery enhances the understanding and optimization of CPN-based gene therapy.