[Irradiation effect of 60Co gamma ray on Aspergillus flavus spores in normal saline and maize]
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Updated: May 31, 2026

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.
Published on: February 15, 2019
Kai Zhong1, Xiang Gao, Rong Ji
1Institute for Nutrition and Food Safety, Chinese CDC, Beijing, China. billyzhongkai@163.com
Objective:
To observe irradiation effect of gamma ray on Aspergilllus flavus spores.
Methods:
Aspergillus flavus spores of different concentration were prepared on two media and were exposed to gamma ray. D10 values and theoretical minimal sterilizing doses were calculated by colony counting results. Actual minimal sterilizing doses were verified according to theoretical dose.
Results:
D10 value and minimal sterilizing dose of high concentration spore in normal saline were 0.39 kGy and 2.5 kGy. Those of low concentration spore in normal saline were 0.47 kGy and 1.5 kGy. Dl0 value and minimal sterilizing dose of high concentration spore in maize were 0.67 kGy and 3.0 kGy. Those of low concentration spore in maize were 0.72 kGy and 2.0 kGy.
Conclusion:
3.0 Gy may be suitable for grain processing to sterilize Aspergillus flavus.

