Related Experiment Video
Updated: May 31, 2026
In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells
Published on: September 24, 2020
[Biological change of normal human breast epithelial cells induced by B( a )P and NNK]
1Institute of Nutrition and Food Safety, Chinese CDC, Beijing 100050, China. xiaoyujoyce@gmail.com
Objective:
To explore the changes of biological characters of normal human epithelial cells treated chronicly with carcinogens.
Method:
At picomolar concentrations of NNK and B [ a ] P carcinogens, noncancerous MCF10A cells were repeatedly treated. Properties of biological target endpoint were studied in cells after 20 cycle's treatment.
Results:
It was found that the treatment can induce cell acquisition of properties of reduced dependence on growth factors, anchorage-independent growth,and acinar-conformational disruption.
Conclusion:
MCF10A cells can acquire cancer-related properties after long-term,low-concentration treatment with carcinogens.
