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Updated: May 31, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
[Rapid method study of methicillin-resistant Staphylococcus aureus identified by matrix-assisted laser desorption
Zongke Sun1, Wei Zhang, Xiping Chen
1Institute for Environment Hygiene and Health Related Product Safety, Chinese Center for Disease 100021, China. Control and Prevent, Beijing, China. sunzongke@yahoo.com.cn
Objective:
To establish the method on rapid identification of Staphylococcus aureus and the performance of methicillin-resistance.
Methods:
40 isolation strains of staphylococcal were analyzed by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS) and API Staph Ident system, and the performance of methicillin-resistance was tested by Disk Diffusion Susceptibility Testing.
Results:
34 isolation strains were identified by MALDI-TOF-MS as Staphylococcus aureus, and the result was uniform to the API Staph Ident system. 11 isolation strains were identified as methicillin-resistant Staphylococcus aureus (MRSA) and 23 isolation strains were methicillin-susceptible to Staphylococcus aureus (MSSA). All the 34 isolation strains were clustered to 2 species identified by MALDI-TOF-MS. The result was uniform to the Disk Diffusion Susceptibility Testing.
Conclusion:
These results demonstrated that MALDI-TOF-MS could be a rapid technique for identification and discrimination of MRSA.
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Rapid Identification of Pathogens
Matrix-Assisted Laser Desorption Ionization (MALDI)
MALDI-TOF Mass Spectrometry
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