Related Experiment Video
Updated: May 31, 2026
A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
[Simultaneous determination of 9 phenolic compounds in disinfectant by HPLC]
Yanwei Yang1, Ying Zhu, Huijuan Zhu
1Institute for Environmental Hygiene and Health Related Product Safety, Chinese Center for Disease 100021, China Control and Prevention, Beijing.
Objective:
A high performance liquid chromatography method was established for determination of 9 phenolic compounds in the disinfectant.
Methods:
on a C18 (4.6 mm x 250 mm, 5 microm) chromatography column, use the methol-0.22% sodium heptane solution (pH 3.5) as mobile phase ladder washing, under the following conditions: flow rate 1.0 ml/min, detection wave 280 nm, and the column temperature 25 degrees C.
Results:
In this method, the linear range of each compound was 5 - 200 mg/L and the correlation coefficients was more than 0.9995. The detection limit was 1mg/L. The RSD was less than 4.8% and standard addition recovery varied from 97.6% to 108.2%.
Conclusion:
This method was easy to operate, fast and with high sensitivity. It was suitable for routine analysis for disinfectant and can be used for the quality control of effective compounds in disinfectant.
