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Related Experiment Video

Updated: May 31, 2026

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

Eucalyptus increases ceramide levels in keratinocytes and improves stratum corneum function.

J Ishikawa¹, Y Shimotoyodome, S Chen

¹Biological Science Laboratory, Kao Corporation, 2606 Akabane, Ichikai-machi, Haga, Tochigi 321-3497, Japan. ishikawa.junko1@kao.co.jp

International Journal of Cosmetic Science

|June 24, 2011

Summary

This study identified a Eucalyptus extract that boosts ceramide levels in skin cells and stratum corneum. This extract enhances skin

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Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

Published on: July 14, 2017

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Last Updated: May 31, 2026

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

Published on: July 14, 2017

Area of Science:

Dermatology and Skin Biology
Plant Science
Biochemistry

Background:

Stratum corneum function is crucial for skin barrier integrity.
Ceramides are key lipids within the stratum corneum, essential for its functions.
Identifying natural compounds that enhance ceramide levels is a therapeutic goal.

Purpose of the Study:

To identify a plant extract that improves stratum corneum functions.
To investigate the mechanism by which the extract enhances skin barrier properties.
To determine if Eucalyptus extract can increase ceramide levels and improve skin hydration and barrier function.

Main Methods:

In vitro studies using human keratinocytes.
In vivo studies involving topical application on human subjects with induced dry skin.
Analysis of ceramide levels, biosynthesis pathways, and gene expression.
Identification of active components within the Eucalyptus extract.

Main Results:

Eucalyptus extract significantly increased ceramide levels in keratinocytes and human stratum corneum.
The extract dose-dependently enhanced ceramide biosynthesis, including glucosylceramide and sphingomyelin.
Topical application improved stratum corneum water-holding capacity and barrier function.
Macrocarpal A, a component of the extract, stimulated ceramide synthesis and related enzyme gene expression.

Conclusions:

Eucalyptus extract effectively improves stratum corneum water-holding and barrier functions.
Increased ceramide content in the stratum corneum is a likely mechanism for the observed therapeutic effects.
Macrocarpal A may be the key component responsible for stimulating ceramide synthesis.