Related Experiment Videos
PAF-acether transfer activity in HL-60 cells is induced during differentiation
1Mountain Aquaculture Research Center, Western Carolina University, Cullowhee, NC 28723.
Biochemical and Biophysical Research Communications
|September 14, 1990
Summary
Platelet-activating factor (PAF) transfer activity in HL-60 cells significantly increases with cellular differentiation into granulocytic or macrophagic lineages. This enhanced transfer is specific to biologically active PAF analogs.
Area of Science:
- Biochemistry
- Cell Biology
- Immunology
Background:
- Platelet-activating factor (PAF) is a potent proinflammatory lipid mediator.
- Intermembrane transfer of PAF analogs has been observed in macrophages.
Purpose of the Study:
- To investigate the modulation of PAF analog transfer activity during HL-60 cell differentiation.
- To determine if this transfer activity is specific to biologically active PAF.
Main Methods:
- HL-60 cells were induced to differentiate using dimethyl sulfoxide, dibutyryl cAMP, or phorbol diester.
- Methylcarbamyl-PAF (biologically active) and 2-O-methyl-PAF (cytotoxic, inactive) transfer activities were measured.
- Transfer activity was quantified in undifferentiated and differentiated HL-60 cells.
Main Results:
- Basal methylcarbamyl-PAF transfer activity in undifferentiated HL-60 cells was 0.56 U.min-1.mg-1.
- Granulocytic differentiation increased methylcarbamyl-PAF transfer activity 2.6-fold.
- Macrophagic differentiation increased methylcarbamyl-PAF transfer activity 6.7-fold.
- Transfer of the inactive analog 2-O-methyl-PAF remained low and unchanged during differentiation.
Conclusions:
- HL-60 cell differentiation significantly enhances the intermembrane transfer of biologically active PAF analogs.
- This enhanced transfer is specific to PAF molecules possessing biological activity.
- The findings suggest a role for PAF intermembrane transfer in cellular differentiation processes.