Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 31, 2026

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Published on: October 15, 2019

Single molecule microscopy methods for the study of DNA origami structures.

Victoria Birkedal¹, Mingdong Dong, Monika M Golas

¹iNANO Center, Aarhus University, 8000 Århus C, Denmark. vicb@inano.au.dk

Microscopy Research and Technique

|June 24, 2011

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Spectrum of double heterozygosity in individuals diagnosed with hereditary breast and ovarian cancer.

European journal of cancer (Oxford, England : 1990)·2026

Same author

Light-driven toluene ammoxidation via mixture photocatalyst of halide perovskite Cs<sub>3</sub>Bi<sub>2</sub>Br<sub>9</sub> and TiO<sub>2</sub>.

Science advances·2026

Same author

Sustainable and Scalable Flow Photochemical Conversions Using a Labile Ligand-Assembled Iron Complex.

JACS Au·2026

Same author

Water-Promoted Molar-Level Photocatalysis and Spontaneous Product Separation with Near-Unity Quantum Efficiency.

Journal of the American Chemical Society·2025

Same author

Dual-Emulsifier Coated Photocatalyst for H<sub>2</sub>O<sub>2</sub> Synthesis in Emulsion via Water Oxidation.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Same author

Photocatalytic Partial Water Dissociation by Protonated Carbon Nitride for Hydrogenation Reactions.

Angewandte Chemie (International ed. in English)·2025

Advanced microscopy techniques like AFM, cryo-EM, and FRET reveal intricate details of DNA origami nanostructures. Combining these methods offers unique insights into complex biological nanomaterials and their functions.

Area of Science:

Nanotechnology
Biophysics
Molecular Biology

Background:

Single molecule microscopy is crucial for studying complex DNA structures, including those from DNA origami.
Advanced self-assembled three-dimensional (3D) DNA nanostructures present unique investigation challenges.

Purpose of the Study:

To discuss the strengths of single molecule fluorescence microscopy, atomic force microscopy (AFM), and cryogenic transmission electron microscopy (cryo-EM).
To demonstrate how the interplay of these techniques provides novel insights into advanced biological nanostructures.

Main Methods:

Atomic Force Microscopy (AFM) for size and structure determination.
Cryogenic Transmission Electron Microscopy (cryo-EM) for high-resolution structural analysis.
Förster/fluorescence resonance energy transfer (FRET) spectroscopy to monitor dynamic processes like lid opening.

More Related Videos

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

Published on: September 27, 2019

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

Related Experiment Videos

Last Updated: May 31, 2026

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Published on: October 15, 2019

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

Published on: September 27, 2019

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

Main Results:

AFM and cryo-EM successfully characterized the size and overall structure of a DNA origami 3D box nanostructure.
FRET spectroscopy effectively recorded the controlled lid opening mechanism of the DNA origami box in response to oligonucleotide keys.

Conclusions:

The combined application of AFM, cryo-EM, and FRET spectroscopy offers a powerful, multi-faceted approach to analyzing complex DNA nanostructures.
This integrated methodology yields significant new insights into the structure, conformation, and dynamic behavior of self-assembled biological nanomaterials.