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Related Experiment Video

Updated: May 31, 2026

Preparation and Characterization of Individual and Multi-drug Loaded Physically Entrapped Polymeric Micelles

Preparation and Characterization of Individual and Multi-drug Loaded Physically Entrapped Polymeric Micelles

Published on: August 28, 2015

Characterization, thermal stability studies, and analytical method development of Paromomycin for formulation

Wahid Khan¹, Neeraj Kumar

¹Department of Pharmaceutics, National Institute of Pharmaceutical Education & Research (NIPER), Nagar, India.

Drug Testing and Analysis

|June 24, 2011

Summary

Paromomycin (PM) is stable up to 120°C, but degrades at 130°C, losing biological activity. A new RP-HPLC method accurately quantifies PM in pharmaceutical formulations.

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Area of Science:

Pharmaceutical Sciences
Analytical Chemistry
Microbiology

Background:

Paromomycin (PM), an aminoglycoside antibiotic, lacks comprehensive pharmaceutical formulation data despite its approval for visceral leishmaniasis.
Understanding PM's thermal stability is crucial for developing stable pharmaceutical formulations.

Purpose of the Study:

To determine the thermal stability of Paromomycin (PM).
To develop a novel analytical method for quantifying PM in pharmaceutical formulations.

Main Methods:

Thermoanalytical techniques (DSC, TGA, HSM) and FTIR spectroscopy were used to assess PM stability.
PM samples were heated at various temperatures (100-130°C) for 24 hours.
Biological activity was evaluated using microbiological assays.
A sensitive RP-HPLC method with pre-column derivatization was developed for PM quantification.

Main Results:

Paromomycin demonstrated stability when heated up to 120°C for 24 hours.
At 130°C, PM exhibited degradation, with a significant loss of biological activity (approximately 30%).
The developed RP-HPLC method accurately quantified PM in parenteral dosage forms with high precision (<2% RSD).

Conclusions:

Paromomycin is thermally stable at temperatures up to 120°C but susceptible to degradation at 130°C.
The newly developed RP-HPLC method is suitable for the sensitive, accurate, and precise quantification of PM.
These findings are essential for the successful development of stable Paromomycin pharmaceutical formulations.