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Updated: May 31, 2026

A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction
Published on: August 27, 2019
Versatile method for the detection of covalently bound substrates on solid supports by DART mass spectrometry
Laura M Sanchez1, Matthew E Curtis, Bianca E Bracamonte
1Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, California 95064, USA.
None:
Analysis of substrates directly on solid phase resins without the need for separate cleavage conditions remains an outstanding challenge in the field of solid phase synthesis. We now present the first example of simultaneous cleavage and mass spectrometric analysis of peptides from solid supports using direct analysis in real time (DART) mass spectrometry. We have shown that this method is compatible with a diverse array of solid phase resins and is suitable for analysis of both peptides and organic substrates.
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