Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intrachromosomal recombination in plants.

A Peterhans1, H Schlüpmann, C Basse

  • 1Institute for Plant Sciences, Swiss Federal Institute of Technology, ETH-Zürich, Switzerland.

The EMBO Journal
|November 1, 1990
PubMed
Summary

This study presents molecular evidence for intrachromosomal recombination in plants. Researchers observed the restoration of a functional gene conferring kanamycin resistance in Nicotiana tabacum cells, indicating DNA repair mechanisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Outcomes Analysis of Patients Receiving Local Ablative Therapy for Oligoprogressive Metastatic NSCLC Under First-Line Immunotherapy.

Clinical lung cancer·2024
Same author

[NSCLC and new oncogenic mutations: Diagnosis and perspectives].

Revue des maladies respiratoires·2021
Same author

RADIORYTHMIC: Phase III, Opened, Randomized Study of Postoperative Radiotherapy Versus Surveillance in Stage IIb/III of Masaoka Koga Thymoma after Complete Surgical Resection.

Clinical lung cancer·2021
Same author

Genetic transformation of Brassica campestris var. rapa protoplasts with an engineered cauliflower mosaic virus genome.

Plant molecular biology·2013
Same author

Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species.

Plant cell reports·2013
Same author

Transposable elements as plant transformation vectors for long stretches of foreign DNA.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

Area of Science:

  • Plant Molecular Biology
  • Genetics and Genomics
  • DNA Recombination Mechanisms

Background:

  • Intrachromosomal recombination is a critical process for genome stability and evolution.
  • Understanding recombination in plants is essential for genetic engineering and crop improvement.
  • Previous studies have indicated the occurrence of recombination, but direct molecular evidence in plants was limited.

Purpose of the Study:

  • To provide molecular evidence for intrachromosomal recombination between closely linked DNA repeats in the plant genome.
  • To investigate the frequency and mechanism of gene restoration via recombination in Nicotiana tabacum.
  • To explore the potential for concerted recombination among multiple tandemly arranged DNA units.

Main Methods:

  • Insertion of non-overlapping, complementary deletion derivatives of the neomycin phosphotransferase gene (nptII) into the Nicotiana tabacum genome.

Related Experiment Videos

  • Selection for kanamycin resistance (KanR) to identify cells where the functional marker gene was restored.
  • DNA analysis of KanR clones, including those with multiple tandem recombination units.
  • Recovery and analysis of kanamycin-sensitive seedlings with patches of KanR cells to demonstrate mitotic recombination.

    • Main Results:

    • Restoration of the functional marker gene (nptII) occurred at frequencies between 10^-4 and 10^-6 per proliferating cell clone.
    • Prolonged tissue culture did not enhance the frequency of recombinant kanamycin-resistant (KanR) cell clones.
    • DNA analysis suggested that multiple tandem recombination units tend to undergo concerted recombination.
    • Direct evidence for mitotic recombination in plant tissue was obtained from analyzing seedlings with mosaic KanR sectors.

    Conclusions:

    • Molecular evidence confirms intrachromosomal recombination between closely linked DNA repeats in the plant genome.
    • The study demonstrates gene restoration through recombination in Nicotiana tabacum, providing insights into DNA repair pathways.
    • Findings support the occurrence of mitotic recombination in plants and suggest concerted recombination events in repetitive DNA regions.