Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 31, 2026

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Published on: May 8, 2014

Microfluidic lipid membrane formation on microchamber arrays.

Sadao Ota¹, Hiroaki Suzuki, Shoji Takeuchi

¹Institute of Industrial Sciences, The University of Tokyo, 4-6-1 Komaba, Tokyo 153-8505, Japan.

|June 25, 2011

Summary

Researchers developed a simple method to create free-standing lipid membranes for studying molecular transport. This technique enables quantitative analysis of non-charged molecule movement across membranes driven by proteins.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same authorSame journal

Complex coacervates enable microRNA concentration for direct nanopore detection.

Lab on a chip·2026

Same author

Microfluidics-Based Engineering of Molecular Self-Assembly and Manufacturing for Artificial Cell Systems.

ACS synthetic biology·2026

Same author

Emulsion-Templated Gel Embedding: A Microfluidics-Free Method for Scalable Cell Encapsulation in Hydrogel Microcapsules.

ACS biomaterials science & engineering·2026

Same author

Clinical Outcomes of Peptide Receptor Radionuclide Therapy in Japanese Patients with Metastatic Rectal Neuroendocrine Tumors.

Cancers·2026

Same author

Acoustofluidic Focusing for High-Throughput and Sensitive Analysis of Shape-Encoded Hydrogel Microparticles in Multiplex Immunoassay.

ACS sensors·2026

Same author

Imaging gas in a combustion engine with high-energy X-ray Compton scattering.

Journal of synchrotron radiation·2026

Area of Science:

Biophysics
Membrane Biology
Biochemistry

Background:

Lipid membranes are crucial for cellular function.
Studying membrane transport requires controlled experimental systems.
Existing methods may lack precision or throughput.

Purpose of the Study:

To develop a novel platform for creating free-standing lipid membranes.
To enable quantitative analysis of molecular transport across these membranes.
To investigate the role of membrane proteins in solute transport.

Main Methods:

Formation of free-standing lipid membranes on microchamber arrays (>100).
Control of solute concentration on either side of the membranes.
Quantitative detection of non-charged fluorescent molecule transport using fluorescence microscopy.

More Related Videos

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method

Published on: December 1, 2015

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Published on: July 10, 2016

Related Experiment Videos

Last Updated: May 31, 2026

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Published on: May 8, 2014

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method

Published on: December 1, 2015

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Published on: July 10, 2016

Main Results:

Successfully formed stable, free-standing lipid membranes.
Demonstrated precise control over solute gradients.
Quantitatively measured the transport of non-charged molecules mediated by membrane proteins.

Conclusions:

The microchamber-based platform offers a robust method for studying lipid membranes.
This system facilitates quantitative investigation of membrane protein function.
The platform is suitable for high-throughput screening of membrane transport processes.