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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: May 31, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Analysis of CpG island methylation using rolling Circle amplification (RCA) product microarray.

Hong Zhao¹, Xiaodong Ma, Minli Li

¹State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China.

Journal of Biomedical Nanotechnology

|June 28, 2011

Summary

This summary is machine-generated.

This study introduces a novel rolling circle amplification (RCA) product microarray for DNA methylation analysis. This method enables high-throughput detection of CpG island methylation in genes like P16 and IGFBP7.

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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

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Published on: September 18, 2020

Targeted DNA Methylation Analysis by Next-generation Sequencing

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Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)

Published on: September 26, 2015

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

DNA methylation is a crucial epigenetic modification involved in gene regulation.
Aberrant DNA methylation patterns are associated with various diseases, including cancer.
Accurate and high-throughput methods for methylation analysis are essential for research and diagnostics.

Purpose of the Study:

To develop and validate a novel rolling circle amplification (RCA) product microarray for DNA methylation analysis.
To assess the efficacy of the RCA product microarray for detecting CpG island methylation in tumor-related genes.

Main Methods:

Bisulfite treatment of DNA followed by PCR amplification.
Design of padlock probes specific to methylated and unmethylated CpG sites.
Ligation of probes to form circular templates for rolling circle amplification (RCA).
Fabrication of DNA microarrays using RCA products for methylation detection.

Main Results:

Successful development of an RCA product microarray for DNA methylation analysis.
Accurate detection of CpG island methylation status in the P16 and IGFBP7 genes.
Microarray data validated by methylation-specific PCR analysis.
Demonstrated potential for high-throughput CpG island methylation detection.

Conclusions:

The RCA product microarray is a promising tool for high-throughput DNA methylation analysis.
This method offers a sensitive and efficient approach for studying CpG island methylation.
The technology has potential applications in cancer research and diagnostics.