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An Efficient Method for Selective Desalination of Radioactive Iodine Anions by Using Gold Nanoparticles-Embedded Membrane Filter
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Published on: July 13, 2018

A simple micro-photometric method for urinary iodine determination.

Gabriele Grimm1, Heidelinde Lindorfer, Heidi Kieweg

  • 1Department of Laboratory Medicine, Medical University of Vienna and the General Hospital of the City of Vienna, Vienna, Austria. gabriele.grimm@meduniwien.ac.at

Clinical Chemistry and Laboratory Medicine
|June 28, 2011
PubMed
Summary

A new photometric test accurately measures urinary iodide concentration (UIC) for assessing iodine status and thyroid health. This simple method is faster than traditional techniques, making it suitable for clinical labs.

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Area of Science:

  • Clinical Chemistry
  • Endocrinology
  • Analytical Chemistry

Background:

  • Urinary iodide concentration (UIC) is a key indicator of nutritional iodine status.
  • Accurate UIC measurement is crucial for managing thyroid conditions and radio-iodine therapies.
  • Existing methods for UIC analysis can be time-consuming and require specialized equipment.

Purpose of the Study:

  • To establish a simple and rapid photometric test for measuring urinary iodide concentration (UIC).
  • To provide a practical method for evaluating iodine status in clinical settings.
  • To offer an alternative to complex methods like ICP-MS for routine UIC analysis.

Main Methods:

  • Urine samples from 200 patients were analyzed using a modified Sandell-Kolthoff (SK) reaction.
  • Samples underwent pre-treatment with ammonium persulfate at 95°C.
  • The photometric SK test was validated against inductively coupled plasma mass spectrometry (ICP-MS).

Main Results:

  • The photometric SK test showed excellent correlation with ICP-MS (r=0.962).
  • The method achieved a functional sensitivity of 20 μg/L.
  • Median UIC in patient samples was 110 μg/L, with 90% below 210 μg/L.

Conclusions:

  • The modified SK-test provides accurate UIC measurements in approximately 90 minutes.
  • This photometric method is significantly faster than ICP-MS (27 hours).
  • The test is suitable for clinical laboratories due to its simplicity and use of basic equipment.