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Global transcriptome response in Lactobacillus sakei during growth on ribose
Anette McLeod1, Lars Snipen, Kristine Naterstad
1Nofima Mat AS, Norwegian Institute of Food, Fisheries and Aquaculture Research, Osloveien 1, Ås, NO-1430, Norway. anette.mcleod@nofima.no
Lactobacillus sakei efficiently utilizes ribose over glucose, with its metabolism and transport systems highly regulated at the transcriptional level for optimal carbon source exploitation.
Area of Science:
- Microbiology
- Molecular Biology
- Biochemistry
Background:
- Lactobacillus sakei is crucial for meat and fish fermentation, preserving products.
- Glucose and ribose are primary carbon sources for L. sakei growth on these substrates.
Purpose of the Study:
- Investigate the global transcriptome response of L. sakei to ribose versus glucose.
- Understand the regulatory mechanisms governing carbon source utilization in L. sakei.
Main Methods:
- Whole-genome microarray analysis of L. sakei strains.
- Comparison of gene expression profiles when grown on ribose versus glucose.
Main Results:
- Differential gene expression primarily related to carbohydrate metabolism and transport.
- Upregulation of genes involved in ribose catabolism, phosphoketolase pathway, and nucleoside metabolism.
- Evidence suggests a CcpA-mediated carbon catabolite repression (CCR) mechanism regulating gene expression.
Conclusions:
- Ribose uptake and catabolism in L. sakei are tightly regulated transcriptionally.
- A global regulatory mechanism fine-tunes enzyme expression for efficient carbon source utilization.
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