Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Optical Biosensing09:39

Optical Biosensing

15.9K
Optical biosensors utilize light to detect the binding of a target molecule. These sensors can utilize a label molecule, which produces a measurable signal such as fluorescence, or these sensors can be label-free and use the changes in optical properties, such as refractive index, to sense for the binding of the target molecule. This video introduces both label and label-free optical biosensors, demonstrates their use in the laboratory, and shows some applications of the...
15.9K
Gold Nanoparticle Synthesis13:42

Gold Nanoparticle Synthesis

15.8K
A protocol for synthesizing ~12 nm diameter gold nanoparticles (Au nanoparticles) in an organic solvent is presented. The gold nanoparticles are capped with oleylamine ligands to prevent agglomeration. The gold nanoparticles are soluble in organic solvents such as...
15.8K
Large Area Substrate-Based Nanofabrication of Controllable and Customizable Gold Nanoparticles Via Capped Dewetting05:51

Large Area Substrate-Based Nanofabrication of Controllable and Customizable Gold Nanoparticles Via Capped Dewetting

6.1K
This protocol details a novel nano-manufacturing technique that can be used to make controllable and customizable nanoparticle films over large areas based on the self-assembly of dewetting of capped metal...
6.1K
Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

8.0K
In this work, a protocol for signal enhancement of nanoprobe-based biosensing is presented. The protocol is based on the reduction of chloroauric acid onto the surface of existing nanoprobes that consist of gold, silver, silica or iron-oxide...
8.0K
Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

12.7K
The design and development of an aptamer–gold nanoparticle colorimetric assay for the detection of small molecules for in-the-field applications was examined. Additionally, a smart-device colorimetric application (app) was validated and long-term storage of the assay was established for use in the...
12.7K
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

15.6K
A protocol is provided to select structure-switching aptamers for small molecule targets based on a tunable stringency magnetic bead selection method. Aptamers selected with structure-switching properties are beneficial for assays that require a conformational change to signal the presence of a target, such as the described gold nanoparticle...
15.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering EVs Via ZnBGs-Integrated 3D Dynamic Culture for Type II Diabetic Pressure Ulcer Therapy.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Electrophile-Nucleophile Paired Heteronuclear Dual-Site for Selective CO<sub>2</sub> Photoreduction to Ethanol via Oxygen-Tethered Asymmetric C-C Coupling.

Angewandte Chemie (International ed. in English)·2026
Same author

Tailored Personas for Lung Transplant Recipients' Return-to-Work Experiences: A Secondary Analysis of Qualitative Data.

Patient preference and adherence·2026
Same author

From bioinformatics to bedside: PPBP drives glioblastoma progression under hypoxia via CXCR2/PI3K/Akt signaling and is targetable by Oenothein B.

Cancer cell international·2026
Same author

Unraveling Ligand-Mediated Electron and Proton Transfer in Nitrate Electroreduction by In Situ Bipolar Electrode Mass Spectrometry.

Analytical chemistry·2026
Same author

Unraveling the Reverse Hofmeister Effect of 3,3',5,5'-Tetramethylbenzidine for Anion-Responsive Color-Changing Hydrogels.

Chemical & biomedical imaging·2026

Related Experiment Video

Updated: Jan 20, 2026

Gold Nanoparticle Synthesis
13:42

Gold Nanoparticle Synthesis

Published on: July 10, 2021

15.8K

Gold nanoparticle-based signal amplification for biosensing.

Xiaodong Cao1, Yongkang Ye, Songqin Liu

  • 1State Key Laboratory of Bioelectronics, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.

Analytical Biochemistry
|June 28, 2011
PubMed
Summary
This summary is machine-generated.

Colloidal gold nanoparticles (AuNPs) offer unique optical and electrochemical properties for biotechnology. This review highlights AuNPs as signal amplification labels in advanced biosensing and biorecognition applications.

More Related Videos

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

8.0K
Design and Development of Aptamer&#8211;Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

12.7K

Related Experiment Videos

Last Updated: Jan 20, 2026

Gold Nanoparticle Synthesis
13:42

Gold Nanoparticle Synthesis

Published on: July 10, 2021

15.8K
Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

8.0K
Design and Development of Aptamer&#8211;Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

12.7K

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Colloidal gold nanoparticles (AuNPs) possess unique optical, electrochemical, and catalytic properties.
  • Their ability to act as biological tags and transducers makes them attractive for biosensing.
  • AuNPs can be conjugated with biomolecules while retaining biochemical activity.

Purpose of the Study:

  • To review recent advances in using AuNPs as labels for signal amplification in biosensing.
  • To focus on signal amplification strategies employing AuNPs in biosensing and biorecognition.
  • To highlight optical and electrochemical detection methods utilizing AuNP-based biosensors.

Main Methods:

  • Review of recent scientific literature on AuNP-based biosensing.
  • Analysis of signal amplification strategies in AuNP applications.
  • Focus on optical and electrochemical detection techniques.

Main Results:

  • AuNPs serve as effective labels for signal amplification in biosensing.
  • Various optical and electrochemical methods leverage AuNPs for enhanced detection.
  • Recent trends show increasing sophistication in AuNP-based sensing applications.

Conclusions:

  • AuNPs are versatile materials for signal amplification in biosensing.
  • Their unique properties enable sensitive and specific biorecognition.
  • Continued research promises further advancements in AuNP-driven biosensor technology.