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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

Updated: May 31, 2026

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
15:29

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Published on: July 10, 2010

Recent advances in flow cytometric cell sorting.

Geoffrey W Osborne1

  • 1Queensland Brain Institute/Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Queensland, Australia.

Methods in Cell Biology
|June 28, 2011
PubMed
Summary

Electrostatic cell sorting, combined with flow cytometry, offers flexible, multi-parameter cell separation. Novel techniques like positional and reflective plate sorting enhance cell-based studies and assay formats.

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Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

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Related Experiment Videos

Last Updated: May 31, 2026

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
15:29

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Published on: July 10, 2010

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
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Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

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Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
10:16

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

Area of Science:

  • Cell biology
  • Biotechnology
  • Analytical chemistry

Background:

  • Cell and particle separation is crucial across scientific disciplines.
  • Electrostatic cell sorting, integrated with flow cytometry, provides advanced multi-parameter separation capabilities.
  • This method surpasses traditional techniques by enabling selections based on reporter levels, not just presence or absence.

Purpose of the Study:

  • To detail the applications of single-cell sorting, focusing on index sorting.
  • To introduce two novel sorting techniques: positional sorting and reflective plate sorting.
  • To highlight the advantages of these advanced sorting methods in various research contexts.

Main Methods:

  • Utilizing electrostatic cell sorting coupled with flow cytometry for multi-parameter analysis.
  • Implementing index sorting for precise cell selection.
  • Developing and applying positional sorting for gate-independent cell studies.
  • Introducing reflective plate sorting for streamlined multiwell plate assays.

Main Results:

  • Demonstrated the utility of electrostatic cell sorting for flexible and precise cell separation.
  • Showcased index sorting's effectiveness in detailed cell analysis.
  • Validated positional sorting for cell-based studies requiring no predefined gating.
  • Established reflective plate sorting as an efficient method for sample and collection plate linkage.

Conclusions:

  • Electrostatic cell sorting with flow cytometry is a powerful tool for diverse scientific applications.
  • Index, positional, and reflective plate sorting represent significant advancements in cell separation technology.
  • These techniques offer enhanced flexibility, efficiency, and applicability in cell-based research and diagnostics.