Related Experiment Videos
Using different sorbents for the concentration of enteroviruses
Bayeva LYe1, G A Shirman, V A Ginevskaya
1Institute of Poliomyelitis and Viral Encephalitis, USSR Academy of Medical Sciences, Moscow.
Journal of Hygiene, Epidemiology, Microbiology, and Immunology
|January 1, 1990
Summary
Nitrocellulose filters and macroporous glass (MPG) effectively concentrate enteroviruses, achieving 64-90% virus sorption. Protein solutions enhance elution from these materials, enabling a 10-40-fold concentration of viruses like rotavirus.
Area of Science:
- Virology
- Environmental Science
- Materials Science
Background:
- Enteroviruses pose significant public health concerns, necessitating efficient detection and concentration methods.
- Macroporous glass (MPG) and membrane filters (MF) are filtration materials used for virus concentration.
- Optimizing virus recovery efficiency (R) and concentration factor (CF) is crucial for sensitive detection.
Purpose of the Study:
- To comparatively evaluate the virus concentration efficiency (EC) of MPG and MF for enteroviruses.
- To assess the sorption properties of different filter materials using radiolabeled virus and protein.
- To determine optimal elution conditions for recovered viruses.
Main Methods:
- Comparative analysis of MPG (MPG-1000 VGKh, SO1) and various MF (nitrocellulose, polycapromide) for enterovirus (poliovirus, simian rotavirus SA-11) concentration.
- Radiolabeling of poliovirus and rabbit serum gamma-globulin to quantify sorption and elution.
- Elution experiments using diverse chemical solutions including protein solutions, amino acid mixtures, and chaotropic salts.
Main Results:
- Nitrocellulose filters (Millipore, PNTs) demonstrated superior virus sorption (64-90%) with lower protein sorption (20-24%).
- Protein solutions (triptosophosphate broth, beef extract) were most effective in eluting rotavirus SA-11 from nitrocellulose filters and MPG.
- MPG-1000 VGKh showed higher recovery efficiency (R) and concentration factor (CF) for rotavirus SA-11 compared to SO1.
Conclusions:
- Nitrocellulose filters and MPG are effective sorbents for enterovirus concentration, enabling 10-40-fold increases.
- The choice of eluent significantly impacts virus recovery, with protein solutions being optimal for rotavirus SA-11.
- MPG-1000 VGKh is a promising material for efficient enterovirus concentration.