Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Stem Cell Niche

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

Zygotic Development And Stem Cell Formation

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...

Maintenance of the ES Cell State

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

Source And Potency Of Stem Cells

Source And Potency Of Stem Cells

Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The long noncoding RNA <i>lnc-FAM164A1</i>-ACLY axis promotes pro-inflammatory responses in human primary macrophages: a systems approach.

Frontiers in immunology·2026

Same author

Precision cardiovascular medicine: shifting the innovation paradigm.

Frontiers in science·2025

Same author

Deficiencies in methionine, tryptophan, and niacin remodels intestinal transcriptome and gut microbiota in female mice.

Scientific reports·2025

Same author

A systems approach to target discovery identifies the role of lncRNA-SPANXA2-OT1 in macrophage chemotaxis.

JCI insight·2025

Same author

Editorial: New trends in vascular biology 2024.

Frontiers in cardiovascular medicine·2025

Same author

Transcriptome wide changes in long noncoding RNAs in diabetic ischemic heart disease.

Cardiovascular diabetology·2024

Search research articles

Related Experiment Video

Updated: May 31, 2026

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

Deeply dissecting stemness: making sense to non-coding RNAs in stem cells.

Shizuka Uchida¹, Pascal Gellert, Thomas Braun

¹Max-Planck-Institute for Heart and Lung Research, Bad Nauheim, Germany. Shizuka.Uchida@mpi-bn.mpg.de

Stem Cell Reviews and Reports

|June 28, 2011

Summary

Human tissue regeneration is limited compared to other species. "-Omics" technologies, like transcriptomics and proteomics, help study stem cell differentiation and its regulation by non-coding RNAs.

More Related Videos

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Published on: May 25, 2018

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

Published on: October 28, 2025

Related Experiment Videos

Last Updated: May 31, 2026

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Published on: May 25, 2018

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

Published on: October 28, 2025

Area of Science:

Regenerative Medicine
Developmental Biology
Genomics

Background:

Adult stem cells are crucial for tissue repair, but human regenerative capacity is limited.
Evolutionary reasons for this limitation remain unclear.
Recent advances in -omics technologies offer new insights.

Purpose of the Study:

To review the impact of expression profiling on stem cell research.
To highlight the role of non-coding RNAs in stem cell differentiation.
To understand the balance of signaling pathways in stem cell plasticity.

Main Methods:

Utilizing transcriptomics and proteomics for detailed expression analysis.
Characterizing stem cell activities at various differentiation stages.
Cataloging changes at both mRNA and protein levels during differentiation.

Main Results:

Expression profiling provides snapshots of stem cell behavior.
Cell differentiation is a plastic process, not a fixed lineage.
Non-coding RNAs play a significant role in regulating stem cell differentiation.

Conclusions:

Understanding stem cell differentiation requires profiling mRNA and protein changes.
Expression profiling, especially non-coding RNAs, is vital for regenerative medicine research.
Further research into signaling pathways is needed to enhance human tissue regeneration.