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Related Experiment Videos

Stretch-sensitive channels in developing muscle cells from a mouse cell line.

A Franco1, J B Lansman

  • 1Department of Pharmacology, School of Medicine, University of California, San Francisco 94143-0450.

The Journal of Physiology
|August 1, 1990
PubMed
Summary

Researchers identified calcium-permeable, cation-selective channels in mouse muscle cells during myogenesis. These channels are mechanically and voltage-gated, showing altered activity with gadolinium block.

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Area of Science:

  • Cell Biology
  • Muscle Physiology
  • Ion Channel Research

Background:

  • Myogenesis involves complex cellular processes, including the regulation of ion transport.
  • Understanding ion channel function is crucial for elucidating muscle development and function.

Purpose of the Study:

  • To identify and characterize calcium-permeable, cation-selective channels in mouse C2 muscle cells during in vitro myogenesis.
  • To analyze the gating properties and expression of these channels throughout myogenesis.

Main Methods:

  • Single-channel recordings from cell-attached patches on mouse C2 muscle cells.
  • Analysis of current-voltage (i-V) relations, cation permeability, and channel kinetics.
  • Investigation of channel gating by mechanical stress (suction) and membrane potential changes.

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  • Assessment of gadolinium (Gd) block on channel activity.

    • Main Results:

    • Identified Ca2(+)-permeable, cation-selective channels gated by suction and membrane potential.
    • Channels exhibit linear i-V relations with monovalent cations and are permeable to Li+, Na+, K+, Rb+, and Cs+.
    • Divalent cations like Ca2+ and Ba2+ showed specific conductances and reversal potentials, with a Ca2+/K+ permeability ratio of approximately 2.
    • Channel openings occurred in bursts, with complex closed-time and burst duration kinetics.
    • Suction increased channel open probability linearly with pressure, enhanced at positive potentials.
    • Depolarization modulated channel activity, affecting the slowest closed-time component.
    • Gadolinium inhibited unitary currents in a concentration-dependent manner, suggesting voltage-independent block.

    Conclusions:

    • A novel class of mechanically and voltage-gated, calcium-permeable cation channels is present in mouse C2 muscle cells during myogenesis.
    • These channels play a role in muscle cell differentiation and function.
    • Gadolinium effectively blocks these channels, providing a tool for further investigation.