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Amplified Fluorescence Turn-On Assay for Mercury(II) Detection and Quantification based on Conjugated Polymer and
1Department of Chemical and Biomolecular Engineering, 4 Engineering Drive 4, National University of Singapore, Singapore 117576, Singapore.
Abstract:
An amplified fluorescence turn-on assay for mercury(II) detection and quantification was developed. This method makes use of specific thymine/mercury(II)/thymine coordination to capture Fl-labeled DNA onto NP surface. Addition of a cationic conjugated polymer leads to an amplified Fl signal in solution. A sigmoidal Hg(2+) working curve is obtained at fixed [NP] with a detection limit of 0.1 × 10(-6) M. However, by reducing [Hg(2+) ] and [NP] simultaneously, while maintaining [Hg(2+) ]:[DNA duplex] = 3:1, a linear calibration curve is observed with a detection limit of 5 × 10(-9) M. The CCP-assisted mercury(II) assay shows potential applications in environmental mercury detection and for industrial process control.
