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Updated: May 31, 2026
12:00
A Practical Guide to Phylogenetics for Nonexperts
Published on: February 5, 2014
An hypothesis-driven, molecular phylogenetics exercise for college biology students
Joel D Parker1, Robert E Ziemba, Sara Helms Cahan
1Department of Biology, Arizona State University, Tempe, Arizona 85287. joel.parker@ie-zea.unil.ch.
Summary
This lab exercise uses DNA evidence to explore evolutionary history and model gene transcription and translation. Students analyze Drosophila DNA to understand cellular information use and evolutionary relationships.
Area of Science:
- Evolutionary Biology
- Molecular Biology
- Genetics
Background:
- Understanding evolutionary history relies on analyzing genetic material.
- Gene transcription and translation are fundamental cellular processes central to molecular biology.
Purpose of the Study:
- To develop a laboratory exercise for early college biology students.
- To teach the use of DNA evidence in investigating evolutionary history.
- To model gene transcription and translation processes.
Main Methods:
- Inquiry-based laboratory approach using DNA evidence.
- Polymerase Chain Reaction (PCR) and DNA sequencing techniques.
- Phylogenetic sequence analysis and pen-and-paper modeling of transcription/translation.
Main Results:
- Students investigate evolutionary relationships among Drosophila species using DNA data.
- Practical application of molecular biology techniques in an evolutionary context.
- Successful integration of cellular processes modeling with DNA analysis.
Conclusions:
- The laboratory effectively links DNA analysis to evolutionary history and cellular processes.
- The exercise is adaptable for different educational levels, including high school 'dry lab' versions.
- Provides students with hands-on experience in molecular techniques and evolutionary concepts.
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