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Related Concept Videos

Enzyme-linked Receptors01:00

Enzyme-linked Receptors

Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Enzyme Kinetics01:19

Enzyme Kinetics

Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...

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Related Experiment Video

Updated: May 31, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Student-designed enzyme-linked metabolite assay kits.

D Hancock1, J Johnston, J Dimauro

  • 1University of Sydney, Darlington NSW 2006, Sydney, Australia.

Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology
|June 28, 2011
PubMed
Summary

Students create their own enzyme-linked metabolite assay kits, learning the benefits and limitations of commercial assay systems. This hands-on approach enhances understanding of metabolite measurement and assay design.

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ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
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ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

Published on: October 23, 2009

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Last Updated: May 31, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
10:28

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

Published on: August 17, 2019

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
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ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

Published on: October 23, 2009

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology Education

Background:

  • Commercial assay kits are widely used in molecular biology and biochemistry.
  • Understanding the components and limitations of these kits is crucial for effective research.
  • Students often lack practical experience with assay development and validation.

Purpose of the Study:

  • To design practical sessions for students to understand commercial assay systems.
  • To facilitate hands-on experience in manufacturing and validating enzyme-linked metabolite assay kits.
  • To promote critical thinking about the appropriate use and limitations of pre-packaged assays.

Main Methods:

  • Students manufactured their own enzyme-linked metabolite assay kits.
  • Investigated component roles, optimized conditions, and checked for cross-reactivity.
  • Prepared buffers, standard solutions, instructions, and packaging for their kits.
  • Assayed "real" samples and had kits validated by peers.

Main Results:

  • Students gained practical experience in assay design and troubleshooting.
  • Developed a deeper understanding of the benefits and pitfalls of commercial kits.
  • Reinforced fundamental principles of metabolite measurement.

Conclusions:

  • Manufacturing custom assay kits provides valuable insights into assay development.
  • This pedagogical approach enhances student comprehension of biochemical assay systems.
  • The process fosters critical evaluation skills for using commercial assay kits effectively.