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Related Experiment Video

Updated: May 31, 2026

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
08:17

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

Evaluating nanoparticle sensor design for intracellular pH measurements.

Rikke V Benjaminsen1, Honghao Sun, Jonas R Henriksen

  • 1DTU Nanotech, Department of Micro- and Nanotechnology, Technical University of Denmark, Building 423, 2800 Lyngby, Denmark.

ACS Nano
|June 29, 2011
PubMed
Summary
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Accurate pH measurement in cells is crucial. New triple-labeled nanosensors offer improved pH quantification in endosomes and lysosomes, overcoming previous limitations in cellular studies.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cell Biology

Background:

  • Particle-based nanosensors enable optical fluorescent-based ratiometric pH measurements in living cells.
  • Quantitative, time-resolved intracellular pH measurements in endosomes and lysosomes remain challenging.
  • Improved measurement methodologies are needed for accurate cellular pH monitoring.

Purpose of the Study:

  • To perform time-resolved pH measurements in endosomes and lysosomes using nanoparticle sensors.
  • To highlight the importance of sensor selection for successful pH quantification.
  • To identify key factors in nanosensor design for future development.

Main Methods:

  • Studied two nanoparticle-based sensor systems internalized via endocytosis.
  • Synthesized triple-labeled nanosensors with a broad pH measurement range (3.2-7.0) using dual pH-sensitive fluorophores.

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Last Updated: May 31, 2026

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
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Published on: July 4, 2011

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
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  • Evaluated nanosensor performance for intracellular pH monitoring.

    • Main Results:

    • Demonstrated successful time-resolved pH measurements in endosomes and lysosomes.
    • Showcased the critical role of sensor choice in accurate pH quantification.
    • Identified that using sensors near their measurement limits leads to erroneous results.
    • Triple-labeled nanosensors with a broad range mitigate quantification errors.

    Conclusions:

    • Nanosensor design, particularly measurement range, is crucial for accurate intracellular pH quantification.
    • Triple-labeled nanosensors offer a viable solution for improved pH monitoring in the endosome-lysosome pathway.
    • Future nanosensor development should prioritize broad measurement ranges to enhance reliability.