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Updated: May 31, 2026
Detection and Isolation of Viable Mouse IL-17-Secreting T Cells
Published on: December 18, 2008
A novel monoclonal antibody specific to mouse IL-17
Jianjun Yang1, Liu Hong, Weizhong Wang
1Department of Digestive Surgery, Xijing Hospital of Digestive Diseases, Fourth Military Medical University of China, Xi'an, Shaanxi Province, China.
Abstract:
IL-17 is secreted by T helper (Th) 17 cells, and might play important roles in host defense against extracellular bacterial infection and fungal infection and contribute to the pathogenesis of various autoimmune inflammatory diseases. Here we have, for the first time, generated the monoclonal antibody against the rat IL-17 protein, which is secreted by T helper (Th) 17 cells. Enzyme-linked immunosorbent assay (ELISA) and Western immunoblotting were used to screen the hybridomas. As a result, one MAb named Y11 (IgG1) was characterized, which was effective in detecting the recombinant and the cellular protein. Then the MAb was used to detect the expression of IL-17 in rat intestine graft tissues by immunohistochemical analysis. Thus, Y11 could be considered a useful tool for studies of IL-17 expression and function.
