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Updated: May 31, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
Published on: November 1, 2019
Streptomyces nucleases
Amruta Pramod Joshi1, Sumedha Sharad Deshmukh
1National Chemical Laboratory, Division of Biochemical Sciences, Dr. Homi Bhabha Road, Pashan, Pune, India.
Abstract:
Streptomyces nucleases are widely distributed and multifunctional enzymes acting on both DNA and RNA. They occur extra as well as intracellularly and can be classified under sugar specific and sugar non-specific nucleases. Nucleases play different roles like analytical, biological, and nutritional. They are also used in programmed cell death. Although more than 20 nucleases are reported to date, very little information is available regarding their structure-function relationship, active site based sequence homology, and the probable mechanism of action. This review describes the history, occurrence, localization, production, purification, properties, and applications of Streptomyces nucleases.
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