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The 64th Symposium of the Society for General Physiologists: optogenetics and superresolution microscopy take center

Graham C R Ellis-Davies1, Edward N Pugh

  • 1Department of Neuroscience, Mount Sinai School of Medicine, New York, USA.

The Journal of General Physiology
|June 29, 2011
PubMed
Abstract

No abstract available in PubMed .

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Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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