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Updated: May 31, 2026

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How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
Published on: February 11, 2019
Protein stability in an ionic liquid milieu: on the use of differential scanning fluorimetry
João V Rodrigues1, Vesna Prosinecki, Isabel Marrucho
1Instituto Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. República 127, 2780-756 Oeiras, Portugal.
Physical Chemistry Chemical Physics : PCCP
|June 29, 2011
Abstract:
Protein stability is a major bottleneck in the biotechnological application of ionic liquid-containing solvents, either in the frame of biocatalysis or protein storage. Herein, differential scanning fluorimetry was successfully implemented as a high throughput method to fast scan the impact of a number of cholinium-based ionic liquids on the stability of proteins.

