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Updated: May 31, 2026

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array
07:19

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Published on: September 7, 2018

Integrated microfluidic array plate (iMAP) for cellular and molecular analysis.

Ivan K Dimov1, Gregor Kijanka, Younggeun Park

  • 1Biomedical Diagnostics Institute, NCSR, Dublin City University, Glasnevin, Dublin, 9, Ireland.

Lab on a Chip
|June 29, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces the integrated microfluidic array plate (iMAP), a novel platform for parallel gene expression, protein immunoassay, and cytotoxicity analysis. The iMAP enables comprehensive cellular and molecular insights from scarce cell samples.

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Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics
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Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics

Published on: March 18, 2021

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Molecular Diagnostics

Background:

  • The Petri dish has been a cornerstone of biomedical science, but modern research demands more integrated cellular analysis platforms.
  • Existing microfluidic platforms often lack the capacity for simultaneous multi-omic analysis (gene, protein, cytotoxicity).
  • A complete understanding of cellular responses requires integrated analysis of gene expression, protein levels, and cell viability.

Purpose of the Study:

  • To present a novel integrated microfluidic array plate (iMAP) for parallel cellular and molecular analysis.
  • To enable simultaneous gene expression, protein immunoassay, and cytotoxicity measurements on a single platform.
  • To facilitate dynamic studies of scarce cell populations by offering low cell number and multi-analyte capabilities.

Main Methods:

  • Development of an integrated microfluidic array plate (iMAP) with on-board gravity-driven flow and open-access fluid exchange.
  • Implementation of a sedimentation-based cell capture mechanism achieving ~100% capture rates.
  • Integration of systems for cell processing, perfusion culture, perturbation, and simultaneous real-time optical analysis.

Main Results:

  • The iMAP platform successfully integrates gene expression, protein immunoassay, and cytotoxicity analysis in parallel.
  • The device demonstrates straightforward operation, handling dilute samples (as few as 5 cells) and low reagent volumes (50 nL).
  • High cell capture efficiency (~100%) and single-cell measurement capabilities for protein and gene expression were achieved.

Conclusions:

  • The iMAP represents a significant advancement in microfluidic cell assay technology.
  • This platform enables comprehensive, multi-parametric analysis of cellular responses, particularly valuable for scarce cell samples.
  • The iMAP has the potential to revolutionize modern biology and clinical science by enabling novel dynamic studies.