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Related Concept Videos

Plant Tissue Culture02:57

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.

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Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
10:29

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Published on: March 30, 2018

Variations in BARE-1 insertion patterns in barley callus cultures.

C Evrensel1, S Yilmaz, A Temel

  • 1Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Vezneciler, Istanbul, Turkey.

Genetics and Molecular Research : GMR
|June 29, 2011
PubMed
Summary

Aging barley calli stability was assessed using the inter-retrotransposon amplified polymorphism (IRAP) technique targeting the BARE-1 retrotransposon. Results show genetic alterations in calli during development, indicating instability under culture conditions.

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Area of Science:

  • Plant molecular biology
  • Genetics
  • Biotechnology

Background:

  • Barley (Hordeum vulgare) callus cultures are essential for genetic studies and crop improvement.
  • Understanding the stability of plant calli during prolonged cultivation is crucial for reliable experimental outcomes.
  • Retrotransposons, like BARE-1 in barley, are mobile genetic elements that can influence genome stability.

Purpose of the Study:

  • To investigate the genetic stability of aging barley calli.
  • To evaluate the utility of the inter-retrotransposon amplified polymorphism (IRAP) technique for assessing genomic changes in barley calli.
  • To analyze alterations in the BARE-1 retrotransposon during barley callus development.

Main Methods:

  • Barley (Hordeum vulgare cv. Zafer-160) mature embryos were cultured on MS medium with 3 mg/L 2,4-D for 60 days.
  • The inter-retrotransposon amplified polymorphism (IRAP) technique was employed using ten primers in 25 combinations.
  • Band profiles generated by IRAP were analyzed to assess genetic similarity and detect retrotransposon alterations.

Main Results:

  • A similarity index of 84% was observed between 30-day-old and 45-day-old calli.
  • The similarity index between mature embryos and 45-day-old calli was 75%.
  • Culture conditions induced alterations in the BARE-1 retrotransposon, reflected in distinct IRAP band profiles.

Conclusions:

  • The study demonstrates that barley calli undergo genetic alterations during aging under the tested culture conditions.
  • The IRAP technique is effective for detecting BARE-1 retrotransposon variations in barley callus development.
  • This research provides the first report on using IRAP for investigating barley callus development and stability.