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Related Concept Videos

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Related Experiment Video

Updated: May 31, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
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Published on: May 8, 2021

High-sensitive protein analysis by FESI-CE-MALDI-MS.

Mohammad Reza Pourhaghighi1, Jean-Marc Busnel, Hubert H Girault

  • 1Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

Electrophoresis
|June 29, 2011
PubMed
Summary

This study combines capillary zone electrophoresis (CZE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) for intact protein analysis. The integrated system significantly enhances sensitivity and detection limits for bioanalytical applications.

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Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Proteomics

Background:

  • Capillary zone electrophoresis (CZE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) are established techniques for protein separation and detection.
  • Coupling CE with MALDI-MS offers a powerful platform for analyzing intact proteins.
  • Existing methods require improvements in sensitivity and detection limits for comprehensive bioanalysis.

Purpose of the Study:

  • To assess the potential of coupling CE with MALDI-MS using a novel iontophoretic fraction collection interface.
  • To integrate field-enhanced sample injection for enhanced sensitivity in intact protein analysis.
  • To develop a high-sensitivity bioanalytical platform for intact protein detection.

Main Methods:

  • Utilized capillary zone electrophoresis (CZE) for the separation of intact proteins.
  • Employed a novel iontophoretic fraction collection interface to couple CZE with MALDI-MS.
  • Integrated field-enhanced sample injection as an online preconcentration strategy.

Main Results:

  • Achieved significant improvements in peak height (over 3200-fold) and peak area (over 4800-fold) under optimized conditions.
  • Demonstrated low limit of detections (LODs) ranging from 5 to 10 nM for intact proteins.
  • Successfully coupled CZE and MALDI-MS with enhanced sensitivity and preconcentration.

Conclusions:

  • The combined CZE-MALDI-MS system with an iontophoretic interface and field-enhanced sample injection is highly effective for intact protein analysis.
  • This bioanalytical platform offers substantial improvements in sensitivity and detection limits.
  • The developed method provides a valuable tool for sensitive proteomic studies.