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Related Concept Videos

M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...

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Related Experiment Video

Updated: May 31, 2026

Identification of Kinase-substrate Pairs Using High Throughput Screening
11:13

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

Substrates of mitotic kinases.

Louise N Johnson1

  • 1Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, Oxford OX13QU, UK. louise.johnson@bioch.ox.ac.uk

Science Signaling
|June 30, 2011
PubMed
Summary

Researchers investigated how kinases identify and phosphorylate substrates during cell division. Understanding kinase-substrate specificity and localization is crucial for coordinating mitosis and cell division processes.

Area of Science:

  • Cellular biology
  • Molecular biology
  • Biochemistry

Background:

  • Cellular signaling pathways heavily rely on phosphorylation reactions mediated by kinases.
  • While some kinase-substrate specificity rules are known, a comprehensive understanding of all substrates remains incomplete.
  • Mitosis, a fundamental process of cell division, involves complex regulatory networks.

Purpose of the Study:

  • To elucidate how kinases recognize substrate sequence motifs during mitosis.
  • To identify specific substrates phosphorylated by relevant kinases within the cellular context.
  • To determine the subcellular localization of these phosphorylation events during cell division.

Main Methods:

  • Analysis of kinase-substrate interactions using sequence motif identification.

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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

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Related Experiment Videos

Last Updated: May 31, 2026

Identification of Kinase-substrate Pairs Using High Throughput Screening
11:13

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach
11:11

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Published on: February 21, 2019

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

  • Phosphoproteomic analysis to identify phosphorylated substrates.
  • Subcellular localization studies to pinpoint phosphorylation sites within cellular compartments.

    • Main Results:

    • Identified specific sequence motifs recognized by kinases for substrate specificity.
    • Determined a set of key substrates phosphorylated during mitosis.
    • Demonstrated the importance of subcellular localization in directing kinase activity and substrate phosphorylation.
    • Highlighted the interplay between sequence recognition and spatial cues in regulating phosphorylation.

    Conclusions:

    • Kinase-substrate specificity during mitosis is governed by both sequence motifs and subcellular localization.
    • This study enhances the molecular understanding of event coordination during cell division.
    • Findings underscore the significance of spatial organization in cellular signaling.