In vitro evaluation on the antioxidant capacity of triethylchebulate, an aglycone from Terminalia chebula Retz fruit
Xiuping Chen1, Fangyun Sun, Lifeng Ma
1Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing.
Objectives:
To evaluate the antioxidant and free-radical scavenging activities of triethylchebulate (TCL), an aglycone isolated from the fruit of Terminalia chebula Retz.
Materials And Methods:
Microsomes, mitochondria and red blood cells (RBCs) were isolated from rat liver. The antioxidant capacities were evaluated by determining the inhibitory effects of TCL on lipid peroxidation, hydrogen peroxide (H(2)O(2))-induced RBCs hemolysis and RBCs autoxidative hemolysis. The free-radical scavenging activities were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and 2´,7´-dichlorodihydrofluorescin diacetate (DCFH(2)-DA) assay.
Result:
TCL significantly inhibited FeSO(4)/Cys-induced microsomes lipid peroxidation and protected both H(2)O(2-)-induced RBCs hemolysis and RBCs auto-hemolysis in a dose-dependent manner. Furthermore, TCL demonstrated potent DPPH free-radical scavenging ability with IC(50) at 2.4×10(-5) M. In addition, TCL also moderately suppressed azide-induced mitochondria ROS formation.
Conclusion:
These results demonstrated that TCL was a strong antioxidant and free-radical scavenger, which might contribute to the anti-oxidative ability of Terminalia chebula Retz.
