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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: May 31, 2026

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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Green fluorescent protein: a perspective.

S James Remington1

  • 1Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403-1229, USA. remington@molbio.uoregon.edu

Protein Science : a Publication of the Protein Society
|June 30, 2011
PubMed
Summary
This summary is machine-generated.

This perspective covers green fluorescent protein (GFP) research from 1994-2011, detailing its structure, function, and the development of fluorescent protein biosensors. It highlights contributions to understanding fluorescence mechanisms and coral-derived fluorescent proteins.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biophysics

Background:

  • Green fluorescent protein (GFP) has revolutionized biological imaging and research since its discovery.
  • Understanding the fundamental properties of GFP is crucial for developing advanced imaging tools.

Purpose of the Study:

  • To provide a personal perspective on key developments in green fluorescent protein (GFP) research between 1994 and 2011.
  • To highlight the contributions of the author's research group to the field.
  • To review structure-function relationships, fluorescence mechanisms, and novel fluorescent proteins.

Main Methods:

  • Structural and functional analysis of the GFP polypeptide.
  • Investigation of fluorescence emission mechanisms.
  • Design and development of ratiometric fluorescent protein biosensors.
  • Characterization of coral-derived fluorescent proteins, including photoswitchable variants and chromoproteins.

Main Results:

  • Detailed insights into the structure-function relationships governing GFP fluorescence.
  • Elucidation of the mechanism of fluorescence emission and excited state proton transfer.
  • Successful design of ratiometric biosensors for various cellular applications.
  • Expansion of the fluorescent protein toolkit with novel variants from marine organisms.

Conclusions:

  • Personal research efforts have significantly advanced the understanding and application of GFP and related fluorescent proteins.
  • The development of fluorescent protein biosensors has enabled sophisticated real-time monitoring of biological processes.
  • Continued exploration of coral reef organisms promises further innovation in fluorescent protein technology.