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[Sorting oleaginous yeast by using optical manipulation and Raman spectroscopy].

Zi-Da Li1, Liang Chen, Ling-Jing Meng

  • 1College of Physics Sciences and Technology, Guangxi Normal University, Guilin 541004, China. lizida23@163.com

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|July 1, 2011
PubMed
Summary

Researchers developed a method using Raman spectroscopy and laser tweezers to efficiently sort oil-producing yeast from mixed cultures. This technique enables rapid identification and collection of valuable oleaginous microorganisms for industrial applications.

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Area of Science:

  • Biotechnology
  • Microbiology
  • Spectroscopy

Background:

  • Screening oleaginous microorganisms is crucial for various industrial applications.
  • Existing methods for yeast sorting can be time-consuming and labor-intensive.

Purpose of the Study:

  • To develop and validate an effective method for sorting oleaginous yeast from mixed populations.
  • To utilize Raman spectroscopy and laser tweezers for real-time identification and isolation of target cells.

Main Methods:

  • Raman spectroscopy was employed to acquire spectral data from yeast cells.
  • Preprocessing techniques included background subtraction, smoothing, baseline correction, and normalization.
  • Principal Component Analysis (PCA) was used for feature extraction.
  • Laser tweezers and optical manipulation facilitated real-time sorting based on a developed model.

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  • Sorted cells were confirmed using microbial culture and Sudan black B staining.

    • Main Results:

    • A sorting model was successfully established based on the distinct spectral compositions of oleaginous and non-oleaginous yeast.
    • Real-time identification and collection of oleaginous yeast cells were achieved using the integrated Raman spectroscopy and optical manipulation system.
    • The sorted oleaginous yeast demonstrated high purity, confirmed by subsequent microbiological analysis.

    Conclusions:

    • Raman spectroscopy combined with optical manipulation offers an effective and efficient approach for sorting oleaginous yeast.
    • This technique holds potential for the isolation of other valuable microorganisms for biotechnological purposes.