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Updated: May 31, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

G-quadruplex DNA aptamers generated for systemin.

Tao Bing¹, Tianjun Chang, Xiaojuan Yang

¹Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Bioorganic & Medicinal Chemistry

|July 1, 2011

Summary

This summary is machine-generated.

Researchers developed DNA aptamers that specifically bind to plant peptide hormone systemin. These aptamers recognize a G-quadruplex structure, offering potential for systemin detection and study in plants.

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Published on: May 12, 2023

Area of Science:

Biochemistry
Molecular Biology
Biotechnology

Background:

Specific ligands for bioactive molecules are crucial for biomedical research and applications.
Systemin, a plant peptide hormone, plays vital roles in plant signaling pathways.

Purpose of the Study:

To select and characterize DNA aptamers with high affinity and specificity for systemin.
To investigate the structural basis of aptamer-systemin binding.

Main Methods:

Systematic evolution of ligands by exponential enrichment (SELEX) for aptamer selection.
Secondary structure prediction, truncation-mutation assays, and circular dichroism (CD) spectroscopy for structural analysis.
Equilibrium dissociation constant (K(d)) measurement and specificity assays.

Main Results:

A group of DNA aptamers specifically binding to systemin was successfully selected.
The binding motif was identified as a hairpin structure with a G-rich loop, further characterized as an antiparallel G-quadruplex.
One aptamer, S-5-1, exhibited high affinity (K(d) = 0.5 μM) and specificity for whole systemin.

Conclusions:

DNA aptamers can be developed as specific ligands for plant peptide hormones like systemin.
The G-quadruplex structure is key to the binding interaction between these aptamers and systemin.
The high-affinity aptamer S-5-1 shows potential for applications in systemin detection, separation, and functional studies in plants.