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Updated: May 31, 2026

In Vivo Confocal Microscopy: A Standard Operating Procedure for the Detection of Demodex Mites at the Eyelid Margin
Published on: July 3, 2025
Risk factors and prevalence of Demodex mites in young adults
Andrea Horváth1, Dóra Maja Neubrandt, Á Ghidán
1Institute of Medical Microbiology, Semmelweis University, Budapest, Hungary. horand@net.sote.hu
Abstract:
Demodex mites are ectoparasites often found in follicles of facial skin. Their role in human diseases is under investigation, and a growing number of studies indicated that they contribute to chronic inflammatory conditions of the skin, such as rosacea, blepharitis, otitis externa, alopecia and folliculitis. In our study we tested 96 healthy adults for the presence of Demodex mites. Risk factors influencing presence of mites and skin types of the tested individuals were evaluated. We found Demodex folliculorum or Demodex brevis in 17.7% of the samples, more frequently in males (21.9%) and in older adults (20%). Use of make-up seems to reduce the likelihood of Demodex carriage, while pet ownership, use of shared items and living in close contact with older adults had no significant influence of presence of mites. Demodex positive individuals described their skin to be drier, more prone to erythema, but less for folliculitis compared to Demodex negative subjects.
Insights
Demodex mites, common in facial skin follicles, were found in 17.7% of healthy adults. This study explored risk factors and skin types associated with Demodex folliculorum and Demodex brevis presence.
Area of Science:
- Dermatology
- Parasitology
- Microbiology
Background:
- Demodex mites are facial ectoparasites.
- Their role in skin conditions like rosacea is under investigation.
- Studies suggest Demodex contributes to chronic inflammatory skin diseases.
Purpose of the Study:
- To determine the prevalence of Demodex mites in healthy adults.
- To identify risk factors associated with Demodex presence.
- To evaluate the relationship between Demodex and skin types.
Main Methods:
- Tested 96 healthy adults for Demodex mites (Demodex folliculorum, Demodex brevis).
- Assessed risk factors including demographics, cosmetic use, pet ownership, and living conditions.
- Evaluated skin types and self-reported skin conditions.
Main Results:
- Demodex mites detected in 17.7% of participants.
- Higher prevalence in males (21.9%) and older adults (20%).
- Makeup use correlated with lower Demodex carriage; other factors showed no significant influence.
Conclusions:
- Demodex mites are prevalent in healthy adults, particularly males and older individuals.
- Skin characteristics in Demodex-positive individuals include dryness and erythema proneness.
- Further research is needed to clarify Demodex's role in specific skin conditions.
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