Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 31, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Thrombin-aptamer recognition: a revealed ambiguity.

Irene Russo Krauss1, Antonello Merlino, Concetta Giancola

  • 1Università di Napoli Federico II, Via Cintia, Napoli, Italia.

Nucleic Acids Research
|July 1, 2011
PubMed
Summary

Modified aptamers offer improved stability and affinity for thrombin, a key enzyme in blood coagulation. Understanding their structure provides insights for designing potent anticoagulant drugs.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thioredoxin Reductase as a Target for Antibacterial Gold Compounds in Burkholderia cenocepacia: Disclosing the Molecular Basis of Enzyme Inhibition.

Chembiochem : a European journal of chemical biology·2026
Same author

Tuning Au Reactivity Beyond Canonical Targets: Ligand-Driven Au(I) Metalation of Lysine Residues in Hen Egg White Lysozyme.

Inorganic chemistry·2026
Same author

Testing sewage sludge and landfill leachate in novel biocovers: A soil column experiment to quantify landfill gas abatement.

Waste management (New York, N.Y.)·2026
Same author

A Molecular Hybrid for Double Stepwise Nitric Oxide Photorelease with Double Fluorescence Readout in Living Cells.

Chemical & biomedical imaging·2026
Same author

Harmonized <sup>1</sup>H NMR Workflow Enables Quantitative Betaine Determination from Nontargeted Metabolite Profiling Using Internal and External Standards<sup>‡‡‡‡‡</sup>.

Analytical chemistry·2026
Same author

Epigenetic mutations: Effects on the stability of G-quadruplex structures in the c-KIT promoter.

International journal of biological macromolecules·2026

Area of Science:

  • Biochemistry
  • Structural Biology
  • Pharmacology

Background:

  • Aptamers are oligonucleotide therapeutics that bind specific molecular targets.
  • The thrombin-binding aptamer (TBA) inhibits thrombin, a critical enzyme in the coagulation cascade.
  • TBA's nuclease susceptibility and limited structural detail hinder its therapeutic potential.

Purpose of the Study:

  • To elucidate the structural basis of thrombin-aptamer interactions.
  • To understand the structural differences between TBA and its modified analog, mTBA.
  • To provide a foundation for rational design of improved aptamer-based anticoagulants.

Main Methods:

  • X-ray crystallography of the thrombin-mTBA complex at 2.15-Å resolution.
  • Structural analysis of aptamer conformation and protein-aptamer interactions.

More Related Videos

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Characterizing Modulators of Protease-Activated Receptors with a Calcium Mobilization Assay Using a Plate Reader
07:13

Characterizing Modulators of Protease-Activated Receptors with a Calcium Mobilization Assay Using a Plate Reader

Published on: May 24, 2024

Related Experiment Videos

Last Updated: May 31, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Characterizing Modulators of Protease-Activated Receptors with a Calcium Mobilization Assay Using a Plate Reader
07:13

Characterizing Modulators of Protease-Activated Receptors with a Calcium Mobilization Assay Using a Plate Reader

Published on: May 24, 2024

  • Comparison of structural data with functional properties of TBA and mTBA.

    • Main Results:

    • The crystal structure reveals detailed thrombin-aptamer interactions.
    • Structural insights explain mTBA's enhanced stability and affinity compared to TBA.
    • The study highlights structural differences correlating with varying inhibitory activities.

    Conclusions:

    • The high-resolution structure of the thrombin-mTBA complex clarifies aptamer-protein binding.
    • Findings elucidate the structural basis for mTBA's improved properties.
    • This research facilitates the rational design of potent aptamer-based anticoagulant drugs.