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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

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Related Experiment Video

Updated: May 31, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

ncFANs: a web server for functional annotation of long non-coding RNAs.

Qi Liao1, Hui Xiao, Dechao Bu

  • 1Bioinformatics Research Group, Key Laboratory of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences, Beijing, PR China.

Nucleic Acids Research
|July 1, 2011
PubMed
Summary

Researchers developed ncFANs, a web tool for annotating long non-coding RNAs (lncRNAs). This service aids in understanding the function of numerous uncharacterized lncRNAs using existing microarray data.

Related Experiment Videos

Last Updated: May 31, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • The non-coding transcriptome is increasingly recognized for its biological significance.
  • Mammalian genomes contain numerous long non-coding RNAs (lncRNAs) requiring functional characterization.
  • Computational approaches are crucial for exploring lncRNA functions.

Purpose of the Study:

  • To develop a user-friendly web service for functional annotation of human and mouse lncRNAs.
  • To facilitate the functional exploration of uncharacterized lncRNAs.
  • To provide a platform for re-using existing microarray data for lncRNA annotation.

Main Methods:

  • Development of ncFANs (non-coding RNA Function ANnotation server), a novel web service.
  • Utilizing re-annotated Affymetrix microarray data for functional annotation.
  • Implementing two annotation strategies: coding-non-coding gene co-expression (CNC) network analysis and condition-related differentially expressed lncRNA identification.

Main Results:

  • ncFANs is the first web service dedicated to functional annotation of human and mouse lncRNAs.
  • The server efficiently re-uses abundant pre-existing microarray data.
  • The current version supports 10 human and mouse Affymetrix microarray platforms with plans for expansion.

Conclusions:

  • ncFANs offers a practical and efficient solution for lncRNA functional annotation.
  • The tool leverages existing data to accelerate research in the non-coding RNA field.
  • Continued updates will enhance the utility of ncFANs with more platforms and lncRNA data.