Inhibited enzyme electrodes. Part 1: Theoretical model
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Updated: Oct 13, 2026

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney
Published on: October 12, 2015
W J Albery1, A E Cass, Z X Shu
1Department of Chemistry, Imperial College, London, UK.
Abstract:
A theoretical model is developed for an electrochemical sensor for toxic substances which works by measuring the inhibition of the enzyme activity. The enzyme is assumed to follow Michaelis-Menten kinetics and the diffusion kinetic equation describing the concentration profile of the enzyme's substrate in the electrolyte layer between the electrode and the membrane covering the electrode is solved. A complete set of analytical solutions is found which corresponds to a number of different rate limiting processes. The set of solutions is described in a case diagram. The use of cytochrome oxidase in particular is discussed.
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