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Quantitative analysis and measurements of near-field interactions in terahertz microscopes

Kiwon Moon1, Euna Jung, Meehyun Lim

  • 1National Laboratory for Nano-THz Photonics, Department of Electrical and Computer Engineering, POSTECH, Pohang, Gyeongbuk, Korea.

Optics Express
|July 1, 2011
PubMed

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.