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Related Experiment Video

Updated: May 31, 2026

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Published on: May 18, 2018

NOD dendritic cells stimulated with Lactobacilli preferentially produce IL-10 versus IL-12 and decrease diabetes

Jean N Manirarora¹, Sarah A Parnell, Yoon-Hyeon Hu

¹Department of Microbiology and Immunology, University of Louisville HSC, 319 Abraham Flexner Way, Louisville, KY 40202, USA.

Clinical & Developmental Immunology

|July 1, 2011

Summary

Lactobacilli, not lipoteichoic acid, induced tolerogenic dendritic cells (DCs) in NOD mice. This suggests L. casei may prevent diabetes by promoting IL-10 production, offering therapeutic potential.

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Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

Published on: May 6, 2013

Area of Science:

Immunology
Microbiology
Autoimmune Diseases

Background:

Dendritic cells (DCs) in NOD mice produce high IL-12, promoting IFNγ-producing T cells and diabetes.
Developing strategies to induce tolerogenic DCs is crucial for preventing autoimmune diabetes.

Purpose of the Study:

To investigate the potential of microorganisms, specifically Lactobacilli, in inducing tolerogenic DCs to prevent autoimmune diabetes.
To compare the effects of Lactobacilli and lipoteichoic acid (LTA) on DC cytokine production and their impact on diabetes development in NOD mice.

Main Methods:

NOD mice-derived DCs were stimulated with Lactobacilli (TLR2 agonist) or LTA (Staphylococcus aureus TLR2 agonist).
Cytokine profiles (IL-12, IL-10) of stimulated DCs were analyzed.
The therapeutic potential was assessed by transferring DCs into NOD mice and monitoring diabetes incidence, with intervention using anti-IL-10R antibodies.

Main Results:

LTA-stimulated DCs produced high IL-12 and accelerated diabetes upon transfer.
L. casei-stimulated DCs favored IL-10 over IL-12 production.
Transfer of L. casei-stimulated DCs reduced diabetes incidence, an effect reversed by anti-IL-10R antibodies.

Conclusions:

L. casei induces a tolerogenic DC phenotype in NOD mice, characterized by increased IL-10 production.
The balance of IL-10 and IL-12 production by DCs is a key indicator for identifying potential therapeutic agents against autoimmune diabetes.