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Related Experiment Video

Updated: May 31, 2026

Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice
11:34

Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice

Published on: February 18, 2017

Continuously perfused microbubble array for 3D tumor spheroid model.

Sivaprakash Agastin, Ut-Binh T Giang, Yue Geng

    Biomicrofluidics
    |July 1, 2011
    PubMed
    Summary
    This summary is machine-generated.

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    A novel microfluidic system using polydimethylsiloxane (PDMS) microbubbles enables controlled culture of multicellular tumor spheroids (MCTSs) under physiological flow. This platform enhances drug screening efficiency and reveals increased drug resistance in 3D tumor models.

    Area of Science:

    • Biomedical Engineering
    • Cancer Research
    • Microfluidics

    Background:

    • Multicellular tumor spheroids (MCTSs) are valuable 3D cancer models for drug testing.
    • Current MCTS methods face challenges in parameter control, time, and cost.

    Purpose of the Study:

    • To develop a microfluidic system for controlled MCTS culture under physiological flow.
    • To validate the system for drug screening and assess spheroid drug resistance.

    Main Methods:

    • A microfluidic system with polydimethylsiloxane (PDMS) microbubbles was designed and computationally simulated (COMSOL).
    • Colo205 tumor spheroids were cultured in PDMS microbubbles under perfusion.
    • Cell viability, E-selectin mediated cell sorting, and doxorubicin toxicity were evaluated.

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    Three-Dimensional (3D) Tumor Spheroid Invasion Assay
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    Three-Dimensional (3D) Tumor Spheroid Invasion Assay

    Published on: May 1, 2015

    Related Experiment Videos

    Last Updated: May 31, 2026

    Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice
    11:34

    Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice

    Published on: February 18, 2017

    Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
    16:18

    Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

    Published on: November 20, 2011

    Three-Dimensional (3D) Tumor Spheroid Invasion Assay
    12:19

    Three-Dimensional (3D) Tumor Spheroid Invasion Assay

    Published on: May 1, 2015

    Main Results:

    • The microfluidic system allowed controlled spheroid culture with uniform size.
    • Colo205 spheroids exhibited a threefold increase in doxorubicin resistance compared to 2D cultures.
    • The system demonstrated potential for real-time imaging and high-throughput drug screening.

    Conclusions:

    • The developed microfluidic system offers a controllable and efficient platform for MCTS culture.
    • This technology advances 3D cancer modeling for more accurate drug screening.
    • The findings highlight the increased drug resistance of spheroids in physiological flow conditions.