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Related Concept Videos

Complement System01:27

Complement System

The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a membrane...
Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy01:16

Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy

Helicobacter pylori, a resilient gram-negative bacterium, can thrive in the stomach's harsh, acidic environment. Infection with H. pylori leads to a cascade of events within the stomach lining. One of the critical disruptions caused by this bacterium is the interference with somatostatin production, a hormone responsible for regulating acid secretion. This interference tips the balance, escalating acid secretion and diminishing bicarbonate levels. This imbalance compromises the defensive...
Gastritis II: Pathophysiology01:26

Gastritis II: Pathophysiology

The pathophysiology of gastritis begins with the colonization of the stomach lining by Helicobacter pylori (H. pylori). This bacterium spreads mainly via the oral-oral route through saliva or shared utensils, and can also be transmitted in overcrowded or unhygienic environments through contaminated water, despite its brief survival outside the body.ColonizationOnce ingested, H. pylori enters the stomach and begins colonization by navigating through the mucus layer lining the stomach wall. It...
Peptic Ulcer Disease II: Pathophysiology01:24

Peptic Ulcer Disease II: Pathophysiology

Peptic ulcer disease develops when protective mechanisms of the gastrointestinal mucosa are overwhelmed by harmful factors, leading to localized erosions in the stomach or proximal duodenum. The main causes are Helicobacter pylori infection and chronic use of nonsteroidal anti-inflammatory drugs (NSAIDs).Helicobacter pylori–Induced InjuryBacterial Adaptation and Colonization:H. pylori is a spiral, Gram-negative bacterium adapted to the acidic stomach. and transmitted through oral-oral or...
Peptic Ulcer Disease II: Pathophysiology01:28

Peptic Ulcer Disease II: Pathophysiology

Peptic Ulcer Disease (PUD) is characterized by the development of ulcers in the stomach or duodenal mucosa. Its pathophysiology is complex, involving a balance between damaging and protective elements.
Damaging agents such as Helicobacter pylori, gastric acid, pepsin, and nonsteroidal anti-inflammatory drugs (NSAIDs) can weaken the mucosal defense, allowing hydrogen ions to infiltrate back and harm epithelial cells.
Complementation Tests00:49

Complementation Tests

A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by Edward Lewis in the 1940s while working on fruit flies. He developed the test to identify the location and arrangement of different mutations on chromosomes.
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...

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Updated: May 31, 2026

High Resolution Electron Microscopy of the Helicobacter pylori Cag Type IV Secretion System Pili Produced in Varying Conditions of Iron Availability
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Complementation system for Helicobacter pylori.

Jinmoon Kim1, Sung-Whan Kim, Sungil Jang

  • 1Department of Oral Biology, Oral Science Research Center, BK21 Project, Research Center for Orofacial Hard Tissue Regeneration, Yonsei University College of Dentistry, Seoul, 120-752, Republic of Korea.

Journal of Microbiology (Seoul, Korea)
|July 1, 2011
PubMed
Summary
This summary is machine-generated.

A new complementation system, pKJMSH, was developed for Helicobacter pylori, improving strain transformation efficiency. This advancement broadens the applicability of genetic studies for this important pathogen.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • The pIR203C04 system facilitated Helicobacter pylori complementation but showed limited strain transformation via natural transformation.
  • Previous methods like electroporation worked for multiple strains, but natural transformation, a key genetic tool, was restricted.
  • This limitation hindered broad application in studying H. pylori molecular pathogenesis.

Purpose of the Study:

  • To redesign and improve the H. pylori complementation system for enhanced strain transformation efficiency.
  • To overcome the narrow selection limitations of the previous pIR203C04 system.
  • To develop a versatile tool applicable to a wider range of H. pylori wild-type strains for genetic manipulation.

Main Methods:

  • Utilized the conserved intergenic region between hp0203 and hp0204 for targeted chromosomal integration.
  • Incorporated conserved 3' coding regions of hp0203 and hp0204 along with a chloramphenicol acetyltransferase cassette and multicloning sites.
  • Constructed the new plasmid, pKJMSH, designed for homologous recombination and natural transformation.

Main Results:

  • The redesigned pKJMSH system successfully transformed all tested H. pylori strains (26695, SSI, J99, 7.13, G27) via natural transformation.
  • This demonstrates significantly improved transformation efficiency compared to the previous system.
  • The integration site did not disrupt other genes, ensuring system integrity.

Conclusions:

  • The novel pKJMSH complementation system overcomes previous limitations, enabling efficient genetic manipulation across diverse H. pylori strains.
  • This improved system is highly applicable for investigating molecular pathogenesis in common H. pylori research strains.
  • PKJMSH represents a valuable advancement for H. pylori research, facilitating broader genetic studies.