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Related Concept Videos

Tight Junctions01:29

Tight Junctions

Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...

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Related Experiment Video

Updated: May 31, 2026

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique
06:43

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

Published on: May 26, 2021

Measuring size-dependent permeability of the tight junction using PEG profiling.

Christina M Van Itallie, James M Anderson

    Methods in Molecular Biology (Clifton, N.J.)
    |July 1, 2011
    PubMed
    Summary

    This study introduces a new method to measure how tight junctions control substance passage. It quantifies both pore and leak pathways, offering better insights into epithelial barrier function.

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    Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
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    Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers

    Published on: October 17, 2013

    Related Experiment Videos

    Last Updated: May 31, 2026

    Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique
    06:43

    Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

    Published on: May 26, 2021

    Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
    18:57

    Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers

    Published on: October 17, 2013

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Physiology

    Background:

    • Tight junctions form selective barriers in epithelia and endothelia, controlling paracellular transport.
    • This barrier is modeled with small pores (approx. 4 Å radius) and larger leak pathways.
    • Current permeability studies often lack detailed size-dependent analysis.

    Purpose of the Study:

    • To develop a method for simultaneously measuring size-dependent apparent permeability across tight junctions.
    • To enable quantification of both pore and leak pathways within the tight junction barrier.
    • To provide a more comprehensive understanding of epithelial and endothelial barrier function.

    Main Methods:

    • Utilized a continuous series of polyethylene polymers as tracers.
    • Simultaneously measured the size-dependence of apparent permeability.
    • Quantified contributions of both pore and leak pathways.

    Main Results:

    • Developed a novel method to assess size-dependent permeability.
    • Enabled simultaneous quantification of pore and leak pathways.
    • Provided a detailed characterization of tight junction transport.

    Conclusions:

    • The new method offers a more precise way to study tight junction permeability.
    • Understanding pore and leak pathways is crucial for physiological and pathological studies.
    • This technique advances the study of epithelial and endothelial barrier dynamics.