Related Experiment Video
Updated: May 31, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
[Experimental study of autotrophic denitrification bacteria through bioaugmentation of activated sludge from
Yu-Mei Chang1, Qi Yang, Chun-Bo Hao
1School of Water Resources and Environment, China University of Geosciences, Beijing 100083, China. changyumei-586@163.com
Abstract:
Activated sludge of municipal wastewater treatment plant was domesticated by sulfur as the electron donor under autotrophic. The sludge activity was determined by measuring growth rate of sludge. The removal efficiency of nitrate and sulfate production efficiency were analyzed by continuously measuring the concentration of NO3(-)-N and SO4(2-). When the removal efficiency of nitrate was more than 90%, 16S rRNA genetic libraries were built up to compare their microbial biodiversity. The growth rate of sludge is 0.177 g/(L x d). The relation between concentration of nitrate and time meets first order reaction kinetics. The bacteria in the sludge affiliated with Beta-Proteobacteria, Deta-Proteobacteria, Gamma-Proteobacteria and Unclassified bacteria. Beta-Proteobacteria is the main phylum in the sludge. Bacteria related to Thiobacillus denitrificans from denitrifying bioreactor perform 48.65%. In addition, the bacteria of Denitratisoma sp., Curvibacter sp., Thermomonas sp., Geobacter sp. are existed in the sludge. The study of autotrophic denitrifying bacteria diversity is conducive to optimization of reaction conditions and efficient removal of nitrate.
More Related Videos
15:19Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
06:11Measuring Biomethane Potential of Food Scrap Waste Anaerobically Co-Digested with Waste-Activated Sludge Using Respirometry
Published on: April 26, 2024
Related Concept Videos
Microbial Wastewater Treatment
Bioremediation
Microbes and the Nitrogen Cycle
Biological Treatment of Effluent and Waste Water
Microbial Bioremediation of Hydrocarbons
Metabolism of Chemolithotrophs