Related Experiment Videos
[Effects of oculo-acupuncture therapy on colonic serotonin reuptake transporter expression in rats with irritable
Shi-yi Song1, Yan-jie Wang, De-shan Wang
1Collegeof Basic Medicine, Liaoning University of Chinese Medicine, Shenyang 110032, China.
Objective:
To investigate the effect of oculo-acupuncture therapy (OAT) on the alteration of colonic serotonin reuptake transporter (SERT) expression in rats with irritable bowel syndrome (IBS) so as to explore its underlying mechanism in relieving IBS.
Methods:
Thirty male Wistar rats were randomized into control, IBS model and OAT groups (n = 10/group). IBS model was established by repeated stress stimulation [water-intake inhibition (24 h), warm box keeping (40 degrees C,5 min), electric shock stimulation (30 V, 120 s), forced cool water swimming (14 t, 5 min), 24 h fasting, and tail clamping (180 s)] for 18 days. Oculo-acupuncture stimulation was given to "Xiajiao" (Lower Energizer), "Dachang" (Large Intestine), "Gan" (Liver) and "Pi" (Spleen) areas for 20 min by using filiform acupuncture needles (manipulated 1 min once every 10 min). The treatment was conducted once every 12 h for 7 days. Colonic SERT mRNA and protein expression levels were examined by RT-PCR and Western Blot.
Results:
In comparison with the control group, the expression levels of both colonic SERT mRNA and protein in the model group were down-regulated remarkably (P < 0.05). Compared with the model group, the expression levels of SERT gene and protein in the OAT group were increased remarkably (P < 0.05).
Conclusion:
OAT can increase colonic SERT protein and gene expression in IBS rats, which may play a role in relieving IBS in OAT clinic.
Related Concept Videos
01:23Drugs Affecting GI Tract Motility: Serotonin Receptor Agonists
01:17Drugs for Treatment of Diarrhea-Predominant IBS
Two specific drugs used in the treatment are alosetron (Lotronex) and eluxadoline (Viberzi). Alosetron, a 5-HT3 antagonist, works by slowing the movement of stools in the gut, reducing bowel...
01:23Irritable Bowel Syndrome
01:21Drugs for Treatment of Constipation-Predominant IBS
01:17Irritable Bowel Syndrome I: Introduction
IBS is a chronic condition that can persist over a long period or recur frequently.
The pathogenesis of IBS involves a complex interplay of the following factors:
Altered...
01:30Irritable Bowel Syndrome II: Clinical Features and Diagnostic Evaluation
Irritable Bowel Syndrome (IBS) is classified into subtypes based on the predominant bowel habits as determined by the Bristol Stool Form Scale (BSFS). The subtypes are: