Related Experiment Video
Updated: Jan 29, 2026
The Number e as a Limit
Published on: January 12, 2026
Organization of minicircle genes for guide RNAs in Trypanosoma brucei
V W Pollard1, S P Rohrer, E F Michelotti
1Department of Biochemistry, University of Alabama, Schools of Medicine and Dentistry, Birmingham 35294.
Abstract:
We have identified four T. brucei minicircle sequences that are complementary to cytochrome oxidase III (COIII) edited mRNA sequence and have shown the existence of transcripts from three of these minicircle sequences. These minicircle transcripts potentially serve as guide RNAs (gRNAs) for RNA editing of the COIII transcript. These gRNAs range in size from 55 to 70 nucleotides, are heterogeneous in sequence, and have a 5' terminal triphosphate. The genes for these gRNAs are flanked by imperfect 18 bp repeats separated by approximately 110 bp. Transcription initiates at the first purine within a conserved sequence, 5'-RYA-YA-3', 31 or 32 bp from the upstream inverted repeat. We propose that these 18 bp inverted repeats are important for minicircle gRNA expression in T. brucei.
Related Concept Videos
The Number e as a Limit
Limits at Infinity
Introduction to Limits
Types of Limits I
Limit Laws I
Limiting Reactant