Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comorbidity and Malnutrition as Key Determinants of Mortality in Elderly Haemodialysis Patients: A One-Year Observational Study.

Journal of cachexia, sarcopenia and muscle·2025

Same author

Madelung's disease: A challenging diagnosis.

Semergen·2024

Same author

Factors associated with patient delay in the diagnosis of TB - a study of health-seeking behaviour.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2024

Same author

The genetic landscape of mitochondrial diseases in the next-generation sequencing era: a Portuguese cohort study.

Frontiers in cell and developmental biology·2024

Same author

Delays have dangerous ends: Tuberculosis diagnosis delay in Portugal, a qualitative study.

Pulmonology·2024

Same author

Ten Years of HIV Diagnosis in a Dermatology and Venereology Department: A Retrospective Study on Demographic, Clinical, and Laboratory Characteristics.

Actas dermo-sifiliograficas·2024

Search research articles

Related Experiment Video

Updated: May 31, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Synergetic chemiluminescence and label-free dual detection for developing a hepatitis protein array.

C Pereira¹, A Yalçın, M Cretich

¹Zoiray Technologies Inc., Boston, MA, USA.

Journal of Immunological Methods

|July 2, 2011

Summary

A novel dual detection system combines label-free optical interference and chemiluminescence for protein arrays. This method accurately quantifies protein binding, enhancing diagnostic capabilities for assays like hepatitis B surface antigen detection.

More Related Videos

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Published on: June 14, 2018

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay

Published on: August 8, 2013

Related Experiment Videos

Last Updated: May 31, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Published on: June 14, 2018

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay

Published on: August 8, 2013

Area of Science:

Biotechnology
Analytical Chemistry
Immunodiagnostics

Background:

Protein arrays are crucial for high-throughput biomolecular analysis.
Existing detection methods have limitations in sensitivity or require labels.
A need exists for versatile detection systems combining label-free and established techniques.

Purpose of the Study:

To develop and validate a dual detection system for protein arrays.
To integrate label-free optical interference with chemiluminescence detection.
To assess the system's performance in detecting hepatitis B surface antigen.

Main Methods:

Fabrication of a planar protein array targeting hepatitis B surface antigen.
Quantification of antibody spot densities using optical interference.
Label-free detection of target binding via optical interference.
Dual detection (optical interference and chemiluminescence) with secondary antibodies for lower concentrations.

Main Results:

Optical interference successfully quantified antibody surface densities.
Label-free detection identified target binding at 10 ng/ml.
Dual detection accurately measured binding at 1 ng/ml.
Binding results correlated directly with initial capture probe density.

Conclusions:

The dual detection system offers both the analytical precision of optical interference and the clinical reliability of chemiluminescence.
This integrated approach enhances the utility of protein arrays for sensitive and quantitative diagnostics.
The system demonstrates direct proportionality between binding signal and probe density, ensuring assay accuracy.